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Adaptive Optics Associates Xinetics
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Lytro Inc
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Adaptive Optics Associates Xinetics
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Hamamatsu
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Advanced Microoptic Systems
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Holographix LLC
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Advanced Microoptic Systems
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Adaptive Optics Associates Xinetics
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Adaptive Optics Associates Xinetics
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Micro-optics Inc
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Image Search Results
Journal: eLife
Article Title: Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy
doi: 10.7554/eLife.84853
Figure Lengend Snippet: ( A ) Schematics of AO-2PFM. Inset 1: direct wavefront measurement by a Shack-Hartmann (SH) sensor composed of a lenslet array and a camera. Inset 2: wavefront correction with a deformable mirror composed of 163 segments with piston, tip, and tilt controls. Grey dashed box: eye imaging module. Bottom: 3D assembly of eye imaging module. L, lens; D, dichroic mirror; DM, deformable mirror; PMT, photomultiplier tube; ETL, electrically tunable lens. ( B ) Maximum intensity projections (MIPs) of image stacks (72×72×25 µm 3 ) of RGC axons measured without and with AO, respectively, normalized to AO image. Insets: k X k Y spatial frequency representation of the images and corrective wavefront. ( C ) MIPs of image stacks (132×97×32 µm 3 ) of fine RGC processes measured without and with AO, respectively, normalized to AO image. ‘No AO’ image brightness artificially increased by 10.6× for better visualization. White arrowheads: putative synaptic structures. Inset: corrective wavefront. Bottom: i : lateral signal profiles along white dashed line i; ii : axial signal profiles of process ii (white arrow). Signals in the line profiles were normalized to the maximal value of the AO condition. Representative data from >3 experiments (technical replicates). Figure 1—source data 1. Source image stacks of retinal axons . ‘1-NoAO_8fAvg_stack.tif’ (No AO) and ‘2-AO_8fAvg_stack.tif’ (AO) Source image stacks of retinal dendrites : ‘1-NoAO_8fAvg_160_100_stack.tif’ (No AO) and ‘2-AO_8fAvg_160_100_stack.tif’ (AO). Figure 1—source data 2. Source image stacks of retinal neuronal processes .
Article Snippet: The SH sensor was composed of a
Techniques: Imaging